OrangerV1, France, Analysis, bibRecord, 000233

Cryopreservation of in vitro grown shoot tips of two interspecific Prunus rootstocks

Identifieur interne : 000233 ( France/Analysis ); précédent : 000232; suivant : 000234

Cryopreservation of in vitro grown shoot tips of two interspecific Prunus rootstocks

Auteurs : Marthe Brison [France] ; Marie-Thérèse De Boucaud [France] ; Françoise Dosba [France]

Source :

Plant Science [ 0168-9452 ] ; 1995.

RBID : ISTEX:689020E5AF7A00CAA030ABCE8B16E4827AF134C0

Abstract

Shoot tips of two in vitro grown interspecific Prunus rootstocks, Fereley-Jaspi (R) and Ferlenain-Plumina (R), were successfully cryopreserved using a two step freezing method. Excised shoot tips from hardened plantlets were precultured for 24 h on solid medium (modified half strength Murashige and Skoog medium (MS)) supplemented with 5% dimethylsulfoxide and 2% proline. They were transferred to 1.2-ml cryotubes and treated with 0.5 ml of modified PVS2 cryoprotective solution for 20–40 min. Cryotubes were then cooled at 1°C/min to −40°C before being immersed in liquid nitrogen (LN2). After 1 min warming at 40°C, shoot tips were rinsed with MS medium containing 1.2 M sucrose and then plated. First regrowth occurred after 5 days and was evaluated 21 days after plating. The average rate of shoot formation was 69% and 74% for Fereley and Ferlenain respectively, compared with 86% for controls of both hybrids. The roles of several factors, especially hardening procedures and meristem size, in the improvement of survival rates, were discussed.

Url:

https://api.istex.fr/document/689020E5AF7A00CAA030ABCE8B16E4827AF134C0/fulltext/pdf

DOI: 10.1016/0168-9452(94)04045-1

Affiliations:

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ISTEX:689020E5AF7A00CAA030ABCE8B16E4827AF134C0

Le document en format XML

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<author><name sortKey="De Boucaud, Marie Therese" sort="De Boucaud, Marie Therese" uniqKey="De Boucaud M" first="Marie-Thérèse" last="De Boucaud">Marie-Thérèse De Boucaud</name>
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<author><name sortKey="Dosba, Francoise" sort="Dosba, Francoise" uniqKey="Dosba F" first="Françoise" last="Dosba">Françoise Dosba</name>
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<front><div type="abstract" xml:lang="en">Shoot tips of two in vitro grown interspecific Prunus rootstocks, Fereley-Jaspi (R) and Ferlenain-Plumina (R), were successfully cryopreserved using a two step freezing method. Excised shoot tips from hardened plantlets were precultured for 24 h on solid medium (modified half strength Murashige and Skoog medium (MS)) supplemented with 5% dimethylsulfoxide and 2% proline. They were transferred to 1.2-ml cryotubes and treated with 0.5 ml of modified PVS2 cryoprotective solution for 20–40 min. Cryotubes were then cooled at 1°C/min to −40°C before being immersed in liquid nitrogen (LN2). After 1 min warming at 40°C, shoot tips were rinsed with MS medium containing 1.2 M sucrose and then plated. First regrowth occurred after 5 days and was evaluated 21 days after plating. The average rate of shoot formation was 69% and 74% for Fereley and Ferlenain respectively, compared with 86% for controls of both hybrids. The roles of several factors, especially hardening procedures and meristem size, in the improvement of survival rates, were discussed.</div>
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Cryopreservation of in vitro grown shoot tips of two interspecific Prunus rootstocks

Cryopreservation of in vitro grown shoot tips of two interspecific Prunus rootstocks

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri